期刊
INSECT SCIENCE
卷 29, 期 1, 页码 215-229出版社
WILEY
DOI: 10.1111/1744-7917.12930
关键词
antioxidant enzyme; embryogenesis; glutathione S-transferase; insecticide resistance; RNAi; Tribolium castaneum
类别
资金
- National Natural Science Foundation of China [31872970, 31572326]
This study investigated the role of GSTS6 in Tribolium castaneum, revealing its significance in antioxidant defense by cooperating with other antioxidant genes and detoxifying insecticides. The findings shed light on the mechanism by which GSTS contributes to antioxidant activity and broaden our understanding of the functional diversity of GSTSs in insects.
The sigma glutathione S-transferases (GSTSs) are a class of cytosolic glutathione S transferases (GSTs) that play important roles in antioxidant defense in insects, but the mechanisms by which GSTSs contribute to antioxidant activity remain unclear. Here, we isolated a GSTS (GSTS6) from Tribolium castaneum and explored its function. Homology and phylogenetic analysis revealed that TcGSTS6 shared high identity with other evolutionarily conserved GSTSs. The recombinant TcGSTS6 protein had strong activity toward cumene hydroperoxide and 4-hydroxynonenal but low activity toward the universal substrate 1-chloro-2,4-dinitrobenzene. Exposure to various types of oxidative stress, including heat, cold, UV and pathogenic microbes, significantly induced TcGSTs6 expression, which indicates that it is involved in antioxidant defense. Knockdown TcGSTs6 by using RNA interference (RNAi) caused reduced antioxidant capacity, which was accomplished by cooperating with other antioxidant genes. Moreover, treatment with various insecticides such as phoxim, lambda-cyhalothrin, dichlorvos and carbofuran revealed that TcGSTS6 plays an important role in insecticide detoxification. The RNAi results showed that TcGSTS6 is essential for embryogenesis in T. castaneum. Our study elucidates the mechanism by which a GSTS contributes to antioxidant activity and enhances our understanding of the functional diversity of GSTSs in insects.
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