Human Saliva as a Diagnostic Specimen for Early Detection of Inflammatory Biomarkers by Real-Time RT-PCR

Nowadays human saliva is more frequently studied as a non-invasive, stress-free, and preferable diagnostic material than blood. Supporting evidences acknowledge saliva as a mirror that reflects the body's physical state. Numerous studies have also demonstrated the presence and use of RNA derived from saliva in the early diagnosis of disease by real-time reverse transcriptase polymerase chain reaction (RT-PCR). Assessing the host inflammatory response in patients and its resolution at an early stage can serve as a prognostic and predictive method in determining therapeutic response or disease progression. In this context, the potential of saliva as a specimen to diagnose early inflammatory biomarkers using RT-PCR seems fascinating and useful. Here, we review inflammatory biomarkers within the saliva, focusing on early detection of these biomarkers using RT-PCR and the factors influencing the quality of saliva specimen.

Automated summary

Saliva is increasingly studied as a non-invasive and stress-free diagnostic material, with RNA extracted from saliva being used for early disease detection through real-time reverse transcriptase polymerase chain reaction (RT-PCR). Assessing the host inflammatory response and its resolution at early stages can serve as a prognostic and predictive method for determining therapeutic response or disease progression. The potential of saliva as a specimen for diagnosing early inflammatory biomarkers using RT-PCR is fascinating and useful.