期刊
FISH & SHELLFISH IMMUNOLOGY
卷 54, 期 -, 页码 40-45出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2016.03.025
关键词
MiR-146a; Inflammatory response; Lipopolysaccharide; Macrophage migration inhibitory factor
资金
- National Natural Science Foundation of China [41206141, 31272635, 31372526]
- Natural foundation of Guangdong Ocean University [1212318]
- Excellent young scientist foundation of Guangdong Ocean University [201402]
Increasing evidence demonstrated that microRNAs (miRNAs) play critical roles in innate immunity in vertebrates and invertebrates. MiR-146a/b is reported as a key regulator of the immune response through mediating Toll-like receptor and cytoldne signalling. In this study, a novel miR-146a was identified and characterised from Pinctada martensii (designated as pm-miR-146a), and its roles in modulating the inflammatory response after LPS stimulation were also investigated. Pm-miR-146a ubiquitously expressed in all examined tissues, with the highest level in the mantle and lowest expression in the haemolymph. Pm-miR-146a increased at 24 h after lipoplysaccharide injection, in union with up regulated NF-kappa B (P < 0.05). The over-expression of pm-miR-146a in vivo could significantly inhibit the expression of macrophage migration inhibitory factor (MIF), the potential target gene predicted by miRanda, while enforcing pm-miR-146a involved in the down-regulation of NF-KB. Thus, we propose that pm-miR-146a plays a role of negative feedback regulation to the NF-KB signal by repressing the expression of the pro-inflammatory cytokine MIF. These findings revealed that miR-146a represents a critical role in inflammatory response and offers new evidence for miRNAs in the innate immunity of molluscs. (C) 2016 Elsevier Ltd. All rights reserved.
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