期刊
FISH & SHELLFISH IMMUNOLOGY
卷 58, 期 -, 页码 96-102出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2016.09.015
关键词
Tachyplesin I; Antiviral effects; Epinephelus coioides; SGIV; RGNNV
资金
- Key Laboratory of Tropical Marine Bio-resources and Ecology [LMB151008]
- National Basic Research Program of China (973 program) [2012CB114402]
- National High Technology Development Program of China (863) [2014AA093507]
- Youth Innovation Promotion Association of the Chinese Academy of Sciences [2015285]
- Science and Technology and industrial development Marine fishery Project of Guangdong Province, China [A201501C01]
- Science and Technology Planning Project of Guangdong Province, China [2014B030301064]
- Key project of natural science research in Anhui Province [KJ2015A154]
- Key support program for Outstanding Young Talents in College of Anhui Province [gxyqZD2016362]
Antimicrobial peptides (AMPs) are ubiquitously found in living organisms and are an important component in innate immune response. Tachyplesin I is a potent antimicrobial peptide isolated from the hemocytes of the horseshoe crab, Tachypleus tridentatus. Previous studies have shown that the 17-residue peptide exhibits a wide spectrum of antimicrobial activity against Gram-negative and Grampositive bacteria, fungi, protozoa, and viruses. However, the efficiencies and defense mechanisms of the Tachyplesin I against fish viruses are still unknown. In this study, Tachyplesin I showed a key role in inhibiting the infection and replication of two kinds of newly emerging marine fish viruses, an enveloped DNA virus of Singapore grouper iridovirus (SGIV), and a non-enveloped RNA virus of viral nervous necrosis virus (RGNNV). Synthetic peptides of Tachyplesin I incubated with virus or cells before infection reduced the viral infectivity. Synthetic peptides of Tachyplesin I drastically decreased SGIV and RGNNV titers and viral gene expression. Grouper spleen (GS) and brain (GB) cells over-expressing Tachyplesin I (GS/pcDNA3.1-flag-Tac I and GB/pcDNA3.1-flag-Tac I) support the inhibition of viral infection. Tachyplesin I activated type I IFN and Interferon-sensitive response element (ISRE) in vitro. The promoter activity of IFN-13 and ISRE were significantly up-regulated in cells transfected with pcDNA3.1-flag-Tac I after infection with SGIV and VNNV. These results suggest that Tachyplesin I is importantly involved in host immune responses to invasion of viral pathogens. (C) 2016 Elsevier Ltd. All rights reserved.
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