期刊
FISH & SHELLFISH IMMUNOLOGY
卷 58, 期 -, 页码 67-72出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2016.09.027
关键词
miR-122; TLR14; miRNA modulate; Toll-like receptor; Immune response
资金
- National Natural Science Foundation of China [31370049, 31672682]
- Natural Science Foundation of Zhejiang Province [LR14C040001]
Innate and acquired immune responses provide critical line in defense against pathogens. MicroRNAs (miRNAs) play crucial roles in regulate gene expression in inflammation and innate immunity. However, the main role of miRNAs are in a large part unclear in aquatic organisms. In this study, we found that miR-122 displayed dramatically declined expression profiles in Vibrio anguillarum challenged miiuy croaker. Meanwhile, we have explored TLR14 as a novel target gene of miR-122 involved in miiuy croakers inflammatory and immune response, which were further estimated through negative expression profiles in both Vibrio anguillarum challenged miiuy croaker and LPS exposure macrophages. Finally, in the dualluciferase reporter assay presented mmi-miR-122 negatively regulated the 3'-UTR of wild-type in luciferase activity rather than the mutant one in HEK-293T cells. This result demonstrated that mmi-miR-122 modulated TLR14 expression by directly targeting TLR14-3'UTR. All of the present data suggested miR-122 was involved in TLR cascade could modulate Vibrio anguillarum infection in miiuy croakers. (C) 2016 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据