期刊
FISH & SHELLFISH IMMUNOLOGY
卷 52, 期 -, 页码 16-22出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2016.03.015
关键词
Crassostrea gigas; Inhibitory neurotransmitter; gamma-aminobutyric acid; Immunomodulation; Immune response
资金
- High Technology Project (863 Program) from Chinese Ministry of Science and Technology [2014AA093501]
- National Science Foundation of China [41276169]
- earmarked fund from Modern Agro-industry Technology Research System [CARS-48]
- Taishan Scholar Program of Shandong
- National & Local Joint Engineering Laboratory of Ecological Mariculture
gamma-aminobutyric acid (GABA) is an inhibitory neurotransmitter to suppress the immune-mediated pro inflammatory reactions, and it has been used in the treatment of many inflammation-related diseases in vertebrates, while its immunomodulatory role in invertebrates has never been reported. In the present study, GABA was found to exist in the hemolymph of Pacific oyster Crassostrea gigas, and its concentration decreased slightly from 8.00 +/- 0.37 mu mol L-1 at normal condition to 7.73 +/- 0.15 mu mol L-1 at 6 h after LPS stimulation, and then increased to 9.34 +/- 0.15 mu mol L-1, 8.86 +/- 0.68 mu mol L-1 at 12 h and 48 h, respectively. After LPS stimulation, the mRNA expressions of pro-inflammatory cytokines (CgIL-17 and CgTNF) and immune effectors (CgSOD and CgBPI), and the protein expression of NOS increased significantly, and these increased trends were remarkably inhibited by GABA stimulation. At the same time, the phagocytosis rate and apoptosis rate of immunocytes also increased obviously after LPS stimulation, whereas the increase was repressed with the addition of GABA. The results collectively demonstrated that GABA was an indispensable inhibitory agent for both humoral and cellular immune response, which mainly functioned at the late phase of immune response to avoid the excess immune reactions and maintain the immune homeostasis. (C) 2016 Elsevier Ltd. All rights reserved.
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