An Electrochemical Nonenzymatic Microsensor Modified by Nickel Cobaltate Nanospheres for Glucose Sensing in Urine

Diabetes is a common chronic disease, so it is particularly important to develop a convenient, fast, and highly sensitive non-enzymatic glucose sensor. In this work, a high sensitivity, low cost sensor was prepared on the sub-strate of printed circuit board. The nickel cobaltate (NiCo2O4) spherical nanomaterials were synthesized by facile hydrothermal and calcining methods. The working electrode was modified by NiCo2O4 nanospheres and the cation exchange resin in turn. Nafion cation exchange film can help to reduce the interference caused by surface-active compounds cyclic voltammetry- amperometric method was used to study the electrocatalytic performance of the sensor at room temperature. The sensor modified by NiCo2O4 nanospheres showed good sensor performance for glucose, including excellent sensitivity (3449.14 mu A mM(-1) cm(-2)), wide linear range (1 mu M to 100 mM), and low detection limit (0.376 mu M). In addition, it has high selectivity, repeatability and stability. The sensor proposed in this work has been successfully applied to the detection of glucose in urine, and the results are consistent with the actual glucose concentration in urine.

Automated summary

A high sensitivity, low cost non-enzymatic glucose sensor was successfully developed on a printed circuit board substrate, showing excellent sensor performance and application prospects.