Difficulty in sex identification of two local populations of red-spotted masu salmon using two salmonid male-specific molecular markers

The present study investigated the validity of two male-specific molecular markers, OtY2 and sdY, in two wild populations of red-spotted masu salmon (Oncorhynchus masou ishikawae). All phenotypic males were positive for both markers in both populations. On the other hand, some phenotypic females were also positive in each population: 32% and 11% in OtY2 and 71% and 67% in sdY. Individuals with no male-specific amplification would be identified as female, suggesting the applicability of these markers (especially, OtY2) when selecting females for life-history studies, strategic conservation, and effective breeding programs.

Automated summary

The study confirmed the validity of two male-specific molecular markers in red-spotted masu salmon populations and suggested the usefulness of these markers, especially OtY2, in selecting females for life-history studies, strategic conservation, and effective breeding programs.