期刊
HUMAN & EXPERIMENTAL TOXICOLOGY
卷 40, 期 12_SUPPL, 页码 S187-S195出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/09603271211036037
关键词
Long non-coding RNA DSCAM-AS1; gastric carcinoma; TPT1; miR-204
类别
资金
- Social Development Projects of Shaanxi Province [2019SF-004]
- National Natural Science Foundation of China [82072722]
The study revealed the overexpression of DSCAM-AS1 in gastric carcinoma, with its silencing resulting in reduced proliferation and migration of GC cells. MiR-204 was identified as a target of DSCAM-AS1, which in turn targeted TPT1 in GC cells. These findings suggest the potential of DSCAM-AS1 as a therapeutic target for preventing GC progression.
Background: Several recent studies have suggested that the long non-coding RNA (lncRNA) DSCAM-AS1 (Down syndrome cell adhesion molecule - anti-sense 1) is aberrantly expressed in many malignancies. Purpose: In this study, we aimed to explore the the role of DSCAM-AS1 in gastric carcinoma. Research Design: Expression of DSCAM-AS1 mRNA, miR-204, and TPT1 (Tumor Protein, Translationally-Controlled 1) were detected using quantitative real-time polymerase chain reaction (qRT-PCR). Proliferation and apoptosis of GC cells were determined using the CCK-8 cell counting assay and flow cytometry. The rate of cell migration and invasion was determined using a transwell assay. The relationships between DSCAM-AS1, miR-204, and TPT1 were predicted and confirmed using a dual-luciferase reporter assay. Expression of TPT1 protein was quantified by Western blot. Results: In this study, we found that DSCAM-AS1 was significantly overexpressed in GC tissues and cell lines. Functional experiments indicated that GC cells with DSCAM-AS1 silencing exhibited a dynamic reduction in proliferation and migration. We identified miR-204 as a target of DSCAM-AS1 and found that it targeted TPT1 in GC cells, which further led to decreased expression of miR-204 in GC tissues and cell lines. A rescue assay revealed that knocked-down DSCAM-AS1 hindered GC progression, which was reversed upon miR-204 downregulation or TPT1 overexpression. Conclusion: We conclude that DSCAM-AS1 is expressed as a tumor oncogene in GC progression, modulated via the miR-204/TPT1 axis. These findings indicate the potential of DSCAM-AS1 as a therapeutic target for GC prevention.
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