Nonlinearity of intracochlear motion and local cochlear microphonic: Comparison between guinea pig and gerbil

Studying the in-vivo mechanical and electrophysiological cochlear responses in several species helps us to have a comprehensive view of the sensitivity and frequency selectivity of the cochlea. Different species might use different mechanisms to achieve the sharp frequency-place map. The outer hair cells (OHC) play an important role in mediating frequency tuning. In the present work, we measured the OHCgenerated local cochlear microphonic (LCM) and the motion of different layers in the organ of Corti using optical coherence tomography (OCT) in the first turn of the cochlea in guinea pig. In the best frequency (BF) band, our observations were similar to our previous measurements in gerbil: a nonlinear peak in LCM responses and in the basilar membrane (BM) and OHC-region displacements, and higher motion in the OHC region than the BM. Sub-BF the responses in the two species were different. In both species the sub-BF displacement of the BM was linear and LCM was nonlinear. Sub-BF in the OHC-region, nonlinearity was only observed in a subset of healthy guinea pig cochleae while in gerbil, robust nonlinearity was observed in all healthy cochleae. The differences suggest that gerbils and guinea pigs employ different mechanisms for filtering sub-BF OHC activity from BM responses. However, it cannot be ruled out that the differences are due to technical measurement differences across the species. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

Studying the in-vivo mechanical and electrophysiological cochlear responses in several species provides a comprehensive understanding of cochlear sensitivity and frequency selectivity. Different species may utilize different mechanisms for sharp frequency tuning, with outer hair cells playing a crucial role in this process. Observations in guinea pigs and gerbils showed similarities in best frequency band responses but differences in sub-best frequency responses, suggesting unique mechanisms for sub-best frequency filtering between the two species. Technical measurement differences could also contribute to these discrepancies.