期刊
GENOMICS
卷 113, 期 4, 页码 2702-2716出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2021.06.004
关键词
Colletotrichum; Stylosanthes; Transcriptomics; Metabolomics; Defense responses; Flavonoid
资金
- Hainan Provincial Natural Science Foundation of China [319QN156]
- National Natural Science Foundation of China [31872409, 31960342, 3181101528]
- Key Research and Development Projects of Hainan Province [ZDYF 2020211]
- China Agriculture Research System of MOF
- Hainan University Startup Fund [KYQD (ZR) 1912]
- Innovation and entrepreneurship project of college students [201910589089]
- China Agriculture Research System of MARA
Our study demonstrates that stylo plants combat Colletotrichum gloeosporioides by up-regulating genes and compounds in the flavonoid biosynthesis pathway, potentially providing targets for resistance breeding.
Colletotrichum, a hemibiotrophic fungal pathogen with a broad host range, causes a yield-limiting disease called anthracnose. Stylo (Stylosanthes) is a dominant pasture legume in tropics and subtropics, and anthracnose is one of its most destructive disease. Resistance mechanisms against anthracnose in stylo are poorly understood, thus hindering the development of resistant varieties. We performed time-resolved leaf transcriptomics, metabolomics and in vitro inhibition assay to investigate the defense responses against Colletotrichum gloeosporioides in stylo. Transcriptomics demonstrated that flavonoid biosynthetic genes were significantly induced during the infection. Consistently, metabolomics also showed the increased accumulation of flavonoid compounds. In vitro assays showed that phloretin and naringenin inhibited the mycelial growth, and apigenin, daidzein, quercetin and kaempferol suppressed conidial germination of Colletotrichum strains. Together, our results suggest that stylo plants cope with C. gloeosporioides by up-regulation of genes and compounds in flavonoid biosynthesis pathway, providing potential targets for resistance breeding.
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