4.6 Article

Upregulation of GBP1 in thyroid primordium is required for developmental thyroid morphogenesis

期刊

GENETICS IN MEDICINE
卷 23, 期 10, 页码 1944-1951

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ELSEVIER SCIENCE INC
DOI: 10.1038/s41436-021-01237-3

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资金

  1. National Science Foundation of China [82070816, 81661168016, 81770786, 81803014, 81870537]
  2. China Postdoctoral Science Foundation [2019M651518, 20161318]
  3. Shanghai Science and Technology Committee [19140904200]

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This study identified four pathogenic GBP1 variants that caused defective thyroid primordium morphogenesis and hypothyroidism in zebrafish embryos. Furthermore, it was found that GBP1 promoted beta-catenin translocation into the cytosol and suppressed the formation of cellular adhesion complexes, leading to defects in thyroid primordium growth.
Purpose Congenital hypothyroidism (CH) is a common congenital endocrine disorder in humans. CH-related diseases such as athyreosis, thyroid ectopy, and hypoplasia are primarily caused by dysgenic thyroid development. However, the underlying molecular mechanisms remain unknown. Methods To identify novel CH candidate genes, 192 CH patients were enrolled, and target sequencing of 21 known CH-related genes was performed. The remaining 98 CH patients carrying no known genes were subjected to exome sequencing (ES). The functions of the identified variants were confirmed using thyroid epithelial cells in vitro and in zebrafish model organisms in vivo. Results Four pathogenic GBP1 variations from three patients were identified. In zebrafish embryos, gbp1 knockdown caused defective thyroid primordium morphogenesis and hypothyroidism. The thyroid cells were stuck together and failed to dissociate from each other to form individual follicles in gbp1-deficient embryos. Furthermore, defects were restored with wild-type human GBP1 (hGBP1) messenger RNA (mRNA) except for mutated hGBP1 (p.H150Y, p.L187P) overexpression. GBP1 promoted beta-catenin translocation into the cytosol and suppressed the formation of cellular adhesion complexes. Suppression of cell-cell adhesion restored the thyroid primordium growth defect observed in gbp1-deficient zebrafish embryos. Conclusion This study provides further understanding regarding thyroid development and shows that defective cellular remodeling could cause congenital hypothyroidism.

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