期刊
GENES & DEVELOPMENT
卷 35, 期 15-16, 页码 1190-1207出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.348190.120
关键词
preterm brain injury; neonatal mouse; meningeal leukocytes; single-cell RNA sequencing]
资金
- Swedish Research Council [2015-06276, 2018-02682, 2019-01320]
- Brain Foundation [FO2017-0102]
- Swedish government [ALFGBG-429801, ALFGBG-813291, ALFGBG-718591]
- National Natural Sci-ence Foundation of China [81771418, U1704281]
- Herbert och Karin Jacobssons Stiftelse
- Elisabet Bollan Foundation
- Vinnova [2019-01320, 2018-02682] Funding Source: Vinnova
- Swedish Research Council [2019-01320, 2018-02682, 2015-06276] Funding Source: Swedish Research Council
The study provides a comprehensive transcriptional atlas of neonatal mouse meningeal leukocytes under normal conditions and after perinatal brain injury, highlighting the differences between neonatal and adult border-associated macrophages. The findings show the importance of meningeal leukocytes in early brain development and pathology, with implications for immune responses and inflammatory cascades after brain injury.
The meninges are important for brain development and pathology. Using single-cell RNA sequencing, we have generated the first comprehensive transcriptional atlas of neonatal mouse meningeal leukocytes under normal conditions and after perinatal brain injury. We identified almost all known leukocyte subtypes and found differences between neonatal and adult border-associated macrophages, thus highlighting that neonatal border-associated macrophages are functionally immature with regards to immune responses compared with their adult counterparts. We also identified novel meningeal microglia-like cell populations that may participate in white matter development. Early after the hypoxic-ischemic insult, neutrophil numbers increased and they exhibited increased granulopoiesis, suggesting that the meninges are an important site of immune cell expansion with implications for the initiation of inflammatory cascades after neonatal brain injury. Our study provides a single-cell resolution view of the importance of meningeal leukocytes at the early stage of development in health and disease.
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