期刊
GENE
卷 784, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.gene.2021.145598
关键词
Cattle; Double muscle; (MSTN) Gene; SNPs; PCR-RFLP
资金
- Ministry of Research, Technology, and Higher Education of the Republic of Indonesia through a scheme of Applied Research (Penelitian Terapan-Strategis Nasional) [4313/IT3. L1/PN/2019]
This study investigated single-nucleotide polymorphisms (SNPs) and 11-bp deletions in the coding region of the MSTN gene in Belgian Blue, Peranakan Ongole, and their crossbred cattle. Although four SNPs and an 11-bp deletion were found, they were not able to differentiate between normal and double-muscled phenotypes accurately.
Determining double muscle based on the myostatin (MSTN) gene in Belgian blue (BB), Peranakan Ongole (PO) and BB ? PO crossbred cattle is very important for crossbreeding programs. This study aimed to investigate single-nucleotide polymorphisms (SNPs) and 11-bp deletions in the coding region of the MSTN gene and their relationship with the double-muscled phenotype in BB ? PO crossbred cattle. A total of 86 blood samples were collected from 28 individual BB, 43 individual PO, and 15 individual BB ? PO crossbred cattle. SNPs and indel 11-bp variation in the coding region of the MSTN gene were found using the sequencing method, followed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. The MSTN gene in the coding region was detected in four SNPs found in PO cattle and its crossbreed. However, the four SNPs could not differentiate normal and double-muscled phenotypes although they are polymorphic. Moreover, in this study, an 11-bp deletion in exon 3 of the MSTN gene in BB cattle was found. In this case, by applying the PCRRFLP technique using the restriction enzyme NmuCI (Tsp45I) in indel 11-bp, the genotypes that were successfully observed were +/+, +/del.11, and del.11/del.11.
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