期刊
FOOD RESEARCH INTERNATIONAL
卷 147, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.foodres.2021.110455
关键词
Non-extractable polyphenols; Proanthocyanidins; High-performance thin-layer chromatography; Phenolic profile; Direct analysis in real-time high-resolution; mass spectrometry; Tropical fruit peels
资金
- FSE Program [S2018/BAA-4393]
- Comunidad of Madrid (Spain)
- FEDER Program [S2018/BAA-4393]
- Comunidad of Madrid [CM/JIN/2019-033]
- University of Alcala [CM/JIN/2019-033]
Alkaline hydrolysis is the most efficient method to recover NEPs from fruit peels, and cherimoya peel extracts contain the richest antioxidant NEPs.
Passiflora species, mangosteen, and cherimoya peels are a source of bioactive phenolic compounds. Nevertheless, a significant fraction of polyphenols, called non-extractable polyphenols (NEPs), are retained in the extraction residue after a conventional extraction. Thus, alkaline, acid, and enzymatic-assisted extractions to recover high contents of antioxidant NEPs from the extraction residue of fruit peels, were compared in this work. A highperformance thin-layer chromatography method with UV/Vis detection was developed in order to obtain the phenolic profile for the extracts. The most intense bands were further analyzed by direct analysis in real-time high-resolution mass spectrometry to tentatively identified NEPs in fruit peel extracts. Total phenolic and proanthocyanidin contents and antioxidant capacity of the extracts were measured to carry out a multivariate statistical analysis. Alkaline hydrolysis was the most efficient treatment to recover NEPs from fruit peels as well as a promising treatment to obtain antioxidant extracts along with EAE. Cherimoya peel extracts were the richest in antioxidant NEPs. This work highlights that many NEPs remain on the extraction residue of fruit peels after conventional extraction and are not usually taken into account.
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