4.7 Article

Prevalence and pathogenic potential of Arcobacter spp. isolated from edible bivalve molluscs in Sardinia

期刊

FOOD CONTROL
卷 127, 期 -, 页码 -

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2021.108139

关键词

Virulence genes; Food-borne pathogen; Shellfish; m-PCR; Mediterranean basin

资金

  1. Ministero del Lavoro, della Salute edelle Politiche Sociali
  2. Dipartimento per la Sanita Pubblica Veterinaria, la Nutrizione e la Sicurezza degli Alimenti Research Project [IZS SA 07/14 RC]

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This study assessed the prevalence of Arcobacter spp. and the presence of virulence genes in edible bivalve molluscs from production farms and retail markets in Sardinia, Italy. The findings indicate a potential health risk associated with the consumption of these shellfish, especially when eaten raw or undercooked, highlighting the need for specific and continuous surveillance programs to ensure food safety.
In recent years, Arcobacter spp. has emerged as an important food-borne pathogen. Members of the genus Arcobacter are frequently isolated from foods of various origin, with particular regard of fish and shellfish. However, little epidemiological information is available on the occurrence of the Arcobacter genus and on the virulence potential of strains recovered from edible bivalve molluscs. The present study was aimed to assess the prevalence of Arcobacter spp. in 86 pool samples (59 mussels, 17 oyster and 10 clams) collected from production farms and retail market of Sardinia (Italy). Species identification was conducted by multiplex PCR and PCR-RFLP. In addition, the presence of 9 putative virulence genes (cadF, hecB, pldA, tlyA, cj1349, irgA, mviN, ciaB and hecA) was assessed by PCR assay. The overall prevalence of Arcobacter spp. was 28.8% in mussels, 35.3% in oyster and 40.0% in clams. The most prevalent species were A. cryaerophilus and A. skirrowii detected each in 12.8% of the samples, followed by A. butzleri detected in 10.5% of the samples. The presence of at least one virulence gene was detected in 58.1% of the strains. A. butzleri isolates harbored 8 out of the 9 putative virulence genes (hecB was never detected) while A. cryaerophilus carried 6 virulence genes (cj1349, hecA, mviN, tlyA, hecB and pldA) and A. skirrowii carried 2 virulence genes (cadF and mviN). The present study indicates the potential risk for human health associated to the consumption of edible bivalve molluscs, especially when eaten raw or undercooked. Considered that the bacterial indicator of fecal contami-nation E. coli is not always a good predictor of the presence of Arcobacter spp. in bivalve molluscs, a specific and continuous surveillance programme should be conducted on these type of shellfish farmed in Sardinia.

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