Colloidal gold immunochromatographic test strips for broad-spectrum detection of Salmonella

Outbreaks of Salmonella infections have always attracted world-wide attention in food industry. Therefore, it is particularly important to detect Salmonella. In this study, we aimed to develop a rapid, simple, sensitive, and broad-spectrum colloidal gold immunochromatographic test strip (ICTS) for detecting Salmonella. The prepared monoclonal antibody (mAb) 1B4 was labeled with colloidal gold and used as the capture antibody, as it can pair with itself to form a sandwich detection platform. The sensitivity of the test strip for Salmonella typhimurium was 4 ? 105 CFU/mL with naked eye and the pathogen was detected in 5?15 min. The colloidal gold ICTS can be used to detect 18 stains of Salmonella and there was no cross-reaction with 14 other food-borne pathogens. Salmonella typhimurium, Salmonella paratyphi B, and Salmonella enterica in chicken were detected after 7 h, 7 h and 6 h of incubation, respectively, and the detection limit was as low as 1 CFU/mL. In short, this is important reports of colloidal gold ICTS for detecting so many stains of Salmonella, and this will be a huge improvement for detecting multiple Salmonella in food samples.

Automated summary

The study has developed a rapid, simple, and sensitive colloidal gold immunochromatographic test strip for detecting Salmonella, with the ability to detect multiple strains within minutes, important for food safety.