期刊
FOOD CHEMISTRY
卷 357, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.129745
关键词
Cathepsin L; Microwave; Myofibrillar proteins; Gel; Silver carp
资金
- National Thirteenth Five-Year Plan for Science Technology [2019YFD0902000]
- National Natural Science Foundation of China [31822038]
- Major Science and Technology Planed Program Projects in Xiamen City [3502Z20201032]
- National First-class Discipline Program of Food Science and Technology [JUFSTR20180102]
- Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province
This study compared the effects of microwave heating and water bath heating on Cat L-induced degradation of myofibrillar protein gels, finding that MW heating effectively inhibited Cat L-induced hydrolysis, resulting in higher gel strength and improved gel properties. The results suggested that MW heating could manipulate disulfide bonds and hydrophobic amino acids to create a compact protein network with good gel properties.
This work was aimed to compare the effect of microwave (MW) heating on the cathepsin L (Cat L)-induced degradation of myofibrillar protein (MP) gels with that of water bath (WB) heating. First, Cat L from silver carp was purified and determined to be 45 kDa. The gel strength of the MW-heated MP gels were significantly higher than those of the WB-heated when Cat L was added (P < 0.05). The gel electrophoresis pattern and scanning electron microscopy analysis indicated that MW heating inhibited the Cat L-induced hydrolysis of MP gels. In addition, the number of sulfhydryl groups and surface hydrophobicity of MW-heated gels were lower than those of WB-heated gels when Cat L was added. These results indicated that MW heating could effectively weaken the degradation of Cat L on MP gels by manipulating disulfide bonds and hydrophobic amino acids, resulting in good gel properties and a compact protein network.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据