期刊
FOOD CHEMISTRY
卷 355, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.129633
关键词
Ratiometric fluorescence ELISA; Gold-silver bimetallic nanoclusters; Horseradish peroxidase; Zearalenone
资金
- National Key Research and Development Program of China [2019YFC1604503]
- Senior Talent Scientific Research Initial Funding Project of Jiangsu University [11JDG1147]
- Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education [RDZH2019003]
- National Special Project for Agroproduct Safety Risk Evaluation of China [GJFP2019002]
- Jiangsu Collabrative Innovation Center of Technology and Material of Water Treatment
The study reported a HRP-mediated ratiometric fluorescence ELISA for detecting ZEN based on fluorescence quenching of Au-Ag NCs. The method showed a lower detection limit and potential sensitivity and reliability, making it a potential platform for ZEN detection.
In the presented study, a horseradish peroxidase (HRP)-mediated ratiometric fluorescence enzyme-linked immunosorbent assay (ELISA) for zearalenone (ZEN) was reported based on fluorescence quenching of goldsilver bimetallic nanoclusters (Au-Ag NCs). HRP-antibody was used as a bridge in this immunoassay, linking the ratiometric fluorescence signal to the ZEN concentration. HRP catalyzed the oxidization of o-phenylenediamine in the presence of H2O2, leading to the formation of 2,3-diaminophenazine, which not only delivered a new peak at 580 nm but also quenched Au-Ag NCs fluorescence at 690 nm. Under optimal conditions, the detection limit for the proposed ELISA was 0.017 ng/mL, which was approximately 6.6-fold lower than conventional ELISA. Moreover, analytical performances were evaluated fully including specificity, accuracy, precision, and practicability, and showed that this method provides a potential platform for sensitive and reliable detection of ZEN.
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