Stereoselective metabolomic and lipidomic responses of lettuce (Lactuca sativa L.) exposing to chiral triazole fungicide tebuconazole

In this study, non-targeted and targeted metabolomics/lipidomics studies based on UPLC-QTOF-MS and UPLCMS/MS were carried out to clarify the effects of tebuconazole and its different enantiomers on lettuce metabolites and lipids. Slight enantioselective degradation of tebuconazole was observed and six degradation metabolites were tentatively identified. The endogenous metabolites involved in carbohydrate metabolism, amino acid metabolism, nucleic acid metabolism, phenylpropanoid and flavonoid metabolism, vitamins, and lipid metabolism were significantly affected with enantioselectivity by tebuconazole exposure. Nucleotide metabolism and nicotinic acid metabolic network were significantly activated by the stimulation of tebuconazole. Rac-and (-)-R-tebuconazole caused the down-regulation of soluble sugars and subsequent amino acids and organic acids. Overall, lettuce exposed to tebuconazole was shown to have a significant impact on plant metabolism and lipid metabolism, with notable stereoselectivity. The results showed stereoselective toxicity of tebuconazole and provided a better understanding of its metabolomic and lipidomic effects on lettuce.

Automated summary

This study used non-targeted and targeted metabolomics/lipidomics studies to investigate the effects of tebuconazole and its enantiomers on lettuce metabolism. Results showed significant stereoselectivity in the impact of tebuconazole on plant metabolism, with activation of nucleotide metabolism and nicotinic acid metabolic network. Exposure to tebuconazole led to down-regulation of soluble sugars and subsequent amino acids and organic acids, indicating notable effects on lettuce metabolomics and lipidomics.