期刊
FOOD CHEMISTRY
卷 351, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.129292
关键词
Carbendazim; Aptamer; Gold nanoparticles; Photothermal effect; Thermometer; Pesticides
资金
- National Key Research and Development Program of China [2019YFC1605604]
- China Agriculture Research System [CARS-26]
- National Natural Science Foundation of China [21707109]
A portable and quantitative photothermal assay platform using AuNPs as indicators has been developed to detect CBZ. The presence of CBZ leads to AuNP aggregation and an increase in temperature, making this method user-friendly, cost-effective, and portable for on-site applications.
The detection of carbendazim (CBZ) is important for food safety and human health. However, most current analytical methods require large instruments and highly trained operators. In order to solve this problem, herein, an innovative portable and quantitative photothermal assay platform relying on a thermometer readout for the detection of CBZ has been developed. Gold nanoparticles (AuNPs), which exhibit a strong distance-dependent photothermal effect under specific laser irradiation, were utilized as indicators. The CBZ aptamer was intro-duced to protect AuNPs from salt-mediated aggregation. When CBZ is present, the binding event between CBZ and aptamer leads to the loss of the aptamer protective effect on AuNPs, and AuNP aggregation occurs. Under 650-nm laser irradiation, the increase in temperature associated with an AuNP-dependent photothermal effect is highly related to the CBZ concentration. Having the advantages of user-friendliness, low cost, quick response, and portability, this method has great potential for on-site applications.
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