期刊
FOOD CHEMISTRY
卷 350, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.129142
关键词
Exosomes; IEC-6 cells; Oxidative stress; AMPK; Purine nucleotides
资金
- National Natural Science Foundation of China [21476176]
- National Key Technology R&D Program of China [2015BAD16B01]
- National High Technology Research and Development Program of China [863 Program] [2015AA021002]
- International Science and Technology Cooperation Programs of Anhui [1503062006]
The study shows that bovine milk exosomes can alleviate purine nucleotide catabolism in oxidatively stressed intestinal epithelial cells, improve energy status, and exert protective effects against oxidative stress.
Evidence suggests that dietary depletion of bovine milk exosomes and their cargos causes a loss of circulating microRNAs and a series of health problems. The aim of the current study was to determine whether bovine milk exosomes affect purine nucleotide metabolism and energy metabolism in oxidatively stressed intestinal crypt epithelial cells (IEC-6). Cells were pretreated with exosomes, followed by H2O2 to induce oxidative stress. Reactive oxidative species (ROS) levels, purine nucleotides, purine metabolic key enzyme activities, cell energy status, and AMPK protein expression were analysed. Exosome pretreatment reduced ROS level and the activities of adenosine deaminase and xanthine oxidase induced by H2O2 in cells. Total adenine nucleotides and energy charge were increased with exosome pretreatment, while the AMPK phosphorylation level was downregulated. The results indicated that bovine milk exosomes could attenuate purine nucleotide catabolism and improve energy status in oxidatively stressed IEC-6 cells and exerted protective effects against oxidative stress.
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