期刊
FOOD AND CHEMICAL TOXICOLOGY
卷 155, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2021.112374
关键词
Diabetes; Stachys riederi var. japonica (Miq.); Streptozotocin; Antioxidant; Chemical composition; LC-MS/MS
资金
- Brain Pool Program through the National Research Foundation of Korea (NRF) - Ministry of Science and ICT [2017H1D3A1A01052610]
- National Research Foundation of Korea [2019R1A1055452]
- Kangwon National University [620160122]
This study investigated the chemical composition, antioxidant, and enzyme inhibitory activities of solvent extract and fractions of Stachys riederi var. japonica, as well as the effects of SJ-EAF in STZ induced type 2 diabetic mice. The results showed that SJ-EAF exhibited promising antidiabetic activity with good antioxidant properties and enzyme inhibitory effects without cytotoxicity, enhancing glucose uptake in cells.
This work analysed the chemical composition, antioxidant, and enzyme inhibitory activities of solvent extract (SJ-ME) and fractions (SJ-HF, SJ-EAF, and SJ-MF) of the Stachys riederi var. japonica (Miq.) (SJ). Furthermore, the effect of SJ-EAF in STZ induced type 2 diabetic mice was examined. Among the samples, SJ-EAF exhibited a lower IC50 concentration of 64.2 +/- 0.48 mu g/mL for DPPH and 82.6 +/- 0.09 mu g/mL for ABTS(+). The SJ-EAF concentration of 2.89 +/- 0.03 mu g and 2.27 +/- 0.98 mu g was equivalent to 1 mu g of acarbose mediated enzyme inhibitory effect against alpha-amylase and alpha-glucosidase, respectively. The SJ-EAF did not show cytotoxicity (<80%) to NIH3T3 nor HepG2 cells but enhanced the glucose uptake in the IR-HepG2. LC-MS/MS of SJ-EAF showed the presence of a total of 16 compounds. Among the identified compounds, rosmarinic acid, caffeic acid, oleanolic acid, and ursolic acid showed high catalytic activity of alpha-amylase and alpha-glucosidase. The treatments of SJ-EAF restored the level of blood glucose, body weight, insulin, HDL and mRNA level of IRS1, GLUT2, GLUT4 and Akt whereas it reduced the excess elevation of total cholesterol, total triglycerides, LDL, AST, ALT, ALP, BUN, and creatinine in STZ induced diabetic mice. Overall, the present study concluded that the SJ-EAF exhibited promising antidiabetic activity.
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