Bioprospecting of cyanobacterium in Chilean coastal desert, Geitlerinema sp. molecular identification and pressurized liquid extraction of bioactive compounds

This study presents a new cyanobacterium of the genus Geitlerinema sp. isolated from the Chilean coastal desert as a rich source of bioactive compounds. Phylogenetic tree is shown along with a broad biochemical characterization using conventional and pressurized liquid extraction. Variables as extraction yield, phycobiliproteins, lipids and methyl palmitate( C16:0) were studied by means of two experimental designs based on a response surface methodology (RSM) employing two factorial design 32. Factors as temperature (20-200 degrees C) and percentage of solvents were selected resulting in Factorial Design A with 0-100% (v/v) of ethanol-water and Factorial Design B with 0-100 (v/v) of ethanol-limonene solvents. The main bioactive compounds were total proteins (similar to 52% wt), phycobiliproteins (especially C-phycocyanin) and methyl palmitate (C16:0). Data obtained showed that water andethanol: water (1:1, v/v) improved the extraction yield and phycobiliproteins recovery. Asfor ethanol:limonene extracts, they were better for lipids and methyl palmitate extractions. In general, high temperatures only improved the extraction yield whereas low temperatures favor the extraction of phycobiliproteins. Therefore, results indicated that Geitlerinema sp. could be one of the several cyanobacteria genera that display functional properties for nutritional, pharmaceutical or industrial use as sustainable and cost-effective bioactive compounds in algal biorefinery model. (C) 2021 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.

Automated summary

This study investigated a new cyanobacterium Geitlerinema sp. from the Chilean coastal desert as a rich source of bioactive compounds. The study used response surface methodology to optimize extraction conditions and identified various bioactive compounds with potential nutritional, pharmaceutical, and industrial applications. The results suggest that Geitlerinema sp. has promising functional properties for sustainable and cost-effective bioactive compounds.