Recent Progresses on Biosensors for Escherichia coli Detection

Foodborne illnesses caused by Escherichia coli (E. coli) are causing worldwide morbidity and mortality, threating human health. One way to limit and eliminate E. coli infections is developing fast and sensitive methods for detection. Biosensors have emerged to complement traditional strategies for E. coli detection. This review aims at providing an updated summary of developed biosensors for specific detection of E. coli in the last 5 years. Since biosensor development first initiated with immobilizing biorecognition element on the transducer, this review starts with a brief discussion on the immobilization methods of biorecognition elements on the biosensor surface, followed by a detailed discussion focused on the progresses made in principles and applications of various biorecognition element-based biosensors for E. coli detection. Especially, the limitations of each method using various biorecognition elements are discussed. Furthermore, the advancements of paper-based biosensors are generalized. In addition, a few suggestions and challenges for prospective development of biosensor are also provided.

Automated summary

This review provides an updated summary of biosensors developed for specific detection of E. coli in the last 5 years, starting with immobilization methods of biorecognition elements and focusing on principles and applications of various biorecognition element-based biosensors for E. coli detection. Limitations of each method using different biorecognition elements are discussed, along with advancements in paper-based biosensors and suggestions for prospective development.