期刊
FEBS LETTERS
卷 595, 期 14, 页码 1902-1913出版社
WILEY
DOI: 10.1002/1873-3468.14136
关键词
lipidic cubic phase; membrane protein; nicotine transport; X-ray crystallography
资金
- JSPS/MEXT KAKENHI [JP19K06526, JP18H02405, JP18KK0197, JP19K22395]
- Uehara Memorial Foundation
- Foundation for Nara Institute of Science and Technology
- Noguchi Institute
- Naito Foundation
- Takeda Science Foundation
- G-7 Scholarship Foundation
- Institute for Fermentation (Osaka)
- Yamada Science Foundation
The transporter NtMATE2 is involved in the transportation of nicotine in the tobacco plant root. The crystal structures of NtMATE2 in its outward-facing forms show a bilobate V-shape. Interaction with an unidentified molecule in the C-lobe cavity and conformational transitions of NtMATE2 suggest a movement related to substrate release.
A transporter of the multidrug and toxic compound extrusion (MATE) family, Nicotiana tabacum MATE2 (NtMATE2), is located in the vacuole membrane of the tobacco plant root and is involved in the transportation of nicotine, a secondary or specialized metabolic compound in Solanaceae. Here, we report the crystal structures of NtMATE2 in its outward-facing forms. The overall structure has a bilobate V-shape with pseudo-symmetrical assembly of the N- and C-lobes. In one crystal structure, the C-lobe cavity of NtMATE2 interacts with an unidentified molecule that may partially mimic a substrate. In addition, NtMATE2-specific conformational transitions imply that an unprecedented movement of the transmembrane alpha-helix 7 is related to the release of the substrate into the vacuolar lumen.
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