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A complete proteomic profile of human and bovine milk exosomes by liquid chromatography mass spectrometry

期刊

EXPERT REVIEW OF PROTEOMICS
卷 18, 期 8, 页码 719-735

出版社

TAYLOR & FRANCIS LTD
DOI: 10.1080/14789450.2021.1980389

关键词

Exosomes; bovine; milk; proteomic; mass spectrometry

资金

  1. New Zealand Ministry for Business, Innovation and Employment
  2. New Zealand dairy farmers through DairyNZ [DRCX1302]
  3. Australian Research Council [DP 140103009]
  4. QUT Children's and Adolescent Seed Fund (CAH)
  5. Mead Johnson Nutrition

向作者/读者索取更多资源

The study investigated the proteomic content of milk exosomes from humans and cows, identifying similarities and differences in protein content. A total of 176 and 186 unique proteins were found in human and bovine milk exosomes, respectively, with 53 proteins common to both species.
Background The present study investigates the proteomic content of milk-derived exosomes. A detailed description of the content of milk exosomes is essential to improve our understanding of the various components of milk and their role in nutrition. Methods The exosomes used in this study were isolated as previously described and characterized by their morphology, particle concentration, and the presence of exosomal markers. Human and bovine milk exosomes were evaluated using Information-Dependent Acquisition (IDA) Mass Spectrometry. A direct comparison is made between their proteomic profiles. Results IDA analyses revealed similarities and differences in protein content. About 229 and 239 proteins were identified in the human and bovine milk exosome proteome, respectively, of which 176 and 186 were unique to each species. Fifty-three proteins were common in both groups. These included proteins associated with specific biological processes and molecular functions. Most notably, the 4 abundant milk proteins lactadherin, butyrophilin, perilipin-2, and xanthine dehydrogenase/oxidase were present in the top 20 list for both human and bovine milk exosomes. Conclusion The milk exosome protein profiles we have provided are crucial new information for the field of infant nutrition. They provide new insight into the components of milk from both humans and bovines.

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