期刊
EXPERIMENTAL BIOLOGY AND MEDICINE
卷 246, 期 23, 页码 2522-2532出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/15353702211033812
关键词
Methyl mercury; atherosclerosis; endothelial cells; inflammation; adhesion molecules; cytokine monocyte-endothelial cell interactions
资金
- Giant Steps Research Development Grant from University of North Carolina at Greensboro
Methyl mercury is linked to cardiovascular disease development by inducing monocyte adhesion to endothelial cells and upregulating proinflammatory cytokines. It also activates the NF-kappa B signaling pathway, leading to increased monocyte binding to endothelial cells. Furthermore, methyl mercury causes necrotic cell death at higher concentrations without inducing apoptosis.
Cardiovascular disease is the leading cause of morbidity, mortality, and health care costs in the USA, and around the world. Among the various risk factors of cardiovascular disease, environmental and dietary exposures to methyl mercury, a highly toxic metal traditionally labeled as a neurotoxin, have been epidemiologically linked to human cardiovascular disease development. However, its role in development and promotion of atherosclerosis, an initial step in more immediately life-threatening cardiovascular diseases, remains unclear. This study was conducted to examine the role that methyl mercury plays in the adhesion of monocytes to human microvascular endothelial cells (HMEC-1), and the underlying mechanisms. Methyl mercury treatment significantly induced the adhesion of monocyte to HMEC-1 endothelial cells, a critical step in atherosclerosis, while also upregulating the expression of proinflammatory cytokines interleukin-6, interleukin-8. Further, methyl mercury treatment also upregulated the chemotactic cytokine monocyte chemoattractant protein-1 and intercellular adhesion molecule-1. These molecules are imperative for the firm adhesion of leukocytes to endothelial cells. Additionally, our results further demonstrated that methyl mercury stimulated a significant increase in NF-kappa B activation. These findings suggest that NF-kappa B signaling pathway activation by methyl mercury is an important factor in the binding of monocytes to endothelial cells. Finally, by using flow cytometric analysis, methyl mercury treatment caused a significant increase in necrotic cell death only at higher concentrations without initiating apoptosis. This study provides new insights into the molecular actions of methyl mercury that can lead to endothelial dysfunction, inflammation, and subsequent atherosclerotic development.
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