期刊
EUROPEAN POLYMER JOURNAL
卷 153, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.eurpolymj.2021.110505
关键词
Bioprinting; Bio-ink; Hydrogel scaffold; Cell culture; Two-photon polymerization; Biocompatibility
资金
- National Key R&D Program of China [2016YFA0200500]
- Austrian-Chinese Cooperative RD Projects [GJHZ1720]
A highly biocompatible bio-ink containing a hydrophilic photoinitiator and modified gelatin was used to fabricate 3D hydrogel scaffolds with mouse fibroblast cells via two-photon polymerization. The cells showed vigorous proliferation within the scaffolds after four days of incubation, suggesting great potential for 3D bioprinting.
Bio-inks with living cells encapsulation are core materials for 3D printing of biomimetic cell culture models in vitro. In this study, a highly biocompatible bio-ink containing a hydrophilic benzylidene cyclopentanone photoinitiator (Y4) and a glycidyl methacrylate modified gelatin (Gel-GM) was prepared, and 3D hydrogel scaffolds with normal mouse fibroblast cells (L929) encapsulation were accurately fabricated by two-photon polymerization on a multiphoton confocal scanning laser microscopy. No apparent damage to L929 cells was observed within the laser-exposed region during the fabrication process. After four Days' incubation, the embedded L929 cells showed a vigorous proliferation and occupied most of the available space within the 3D hydrogel scaffolds, which indicated that the bio-ink would have great potential for 3D bioprinting.
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