4.7 Article

A novel visceral pain model of uterine cervix inflammation in rat

期刊

EUROPEAN JOURNAL OF PHARMACOLOGY
卷 900, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.ejphar.2021.174080

关键词

c-Fos; Inflammation; Pain behavior; Pain model; Uterine cervix; Visceral pain

资金

  1. National Natural Science Foundation of China (NSFC) [81870868, 81471126, 81501702]

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This study established a rat model of pain induced by uterine cervix inflammation, with a novel pain score derived from specific behaviors and c-Fos expression in the spinal cord. The highest pain score and c-Fos expression were found in the 0.9 mg capsaicin group. Intrathecal morphine significantly reduced both the pain score and c-Fos expression in the spinal cord.
Treatment of visceral pain originating from the uterine cervix is a substantial clinical problem. The underlying mechanisms of such visceral pain remain unclear mainly due to a lack of reliable model. This study aimed to develop and evaluate the performance of a rat model of pain induced by uterine cervix inflammation. Rats were randomized to six groups according to the solution injected into the uterine cervix: normal saline, vehicle, capsaicin (0.3 mg, 0.6 mg, 0.9 mg), capsaicin 0.9 mg + morphine (n = 15 in each group). Spontaneous behaviors after cervical injection were recorded by a computerized video system and analyzed offline. An equation for calculating a novel pain score was derived from particular behaviors, based on Pearson?s correlation analysis and regression analysis. c-Fos expression in the spinal cord was detected. The pain score and c-fos expression in the spinal cord were highest in the 0.9 mg capsaicin group and lowest in the normal saline and vehicle groups (P < 0.05). Intrathecal morphine significantly decreased the pain score (P < 0.05) and c-fos expression in the spinal cord (P < 0.05). Injection of capsaicin into the uterine cervix in rats could be a practical model of inflammatory cervical pain, which can be evaluated using our novel pain score. This model will provide further insight into the mechanism underlying visceral pain originating from the uterine cervix.

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