期刊
EUROPEAN JOURNAL OF CANCER
卷 150, 期 -, 页码 179-189出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ejca.2021.03.023
关键词
Leukapheresis; Prostatic neoplasms; Circulating neoplastic cells; Organoids; Genetic heterogeneity
类别
资金
- KWF-Alpe d'HuZes project [EMCR 2015e8037]
- Dutch Association of Medical Oncology (NVMO)
DLA is an efficient method to enrich CTCs from patients with mPCa, providing a rich source of viable tumour cells despite the modest success rate in culturing CTCs. Organoid cultures had a success rate of 35%, with significantly higher CTC numbers in successful cultures.
Background: Circulating tumour cell (CTC)-derived organoids have the potential to provide a powerful tool for personalised cancer therapy but are restrained by low CTC numbers provided by blood samples. Here, we used diagnostic leukapheresis (DLA) to enrich CTCs from patients with metastatic prostate cancer (mPCa) and explored whether organoids provide a platform for ex vivo treatment modelling. Methods: We prospectively screened 102 patients with mPCa and performed DLA in 40 patients with >5 CTCs/7.5 mL blood. We enriched CTCs from DLA using white blood cell (WBC) depletion alone or combined with EpCAM selection. The enriched CTC samples were cultured in 3D to obtain organoids and used for downstream analyses. Results: The DLA procedure resulted in a median yield of 5312 CTCs as compared with 22 CTCs in 7.5 mL of blood. Using WBC depletion, we recovered 46% of the CTCs, which reduced to 12% with subsequent EpCAM selection. From the isolated and enriched CTC samples, organoid expansion succeeded in 35%. Successful organoid cultures contained significantly higher CTC numbers at initiation. Moreover, we performed treatment modelling in one organoid cell line and identified substantial tumour heterogeneity in CTCs using single cell DNA sequencing. Conclusions: DLA is an efficient method to enrich CTCs, although the modest success rate of culturing CTCs precludes large scale clinical application. Our data do suggest that DLA and subsequent processing provides a rich source of viable tumour cells. Therefore, DLA offers a promising alternative to biopsy procedures to obtain sufficient number of tumour cells to study sequential samples in patients with mPCa. Trial registration number: NL6019. (c)& nbsp;2021 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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