Morphological, behavioral and genotoxic effects of glyphosate and 2,4-D mixture in tadpoles of two native species of South American amphibians

Pesticide contamination is an important factor in the global decline of amphibians. The herbicides glyphosate and 2,4-D are the most applied worldwide. These herbicides are often found in surface waters close to agricultural areas. This study aims at evaluating the chronic effects caused by glyphosate + 2,4-D mixture in Boana faber and Leptodactylus latrans tadpoles. The combined solution of the glyphosate and 2,4-D, in 5 different concentrations, was applied for 168 h. Herbicide mixtures did not affect the survival of the exposed tadpoles but growth and swimming activity were altered; besides causing several damages in the mouth and intestine. The erythrocytes showed micronuclei and other nuclear abnormalities. There is an ecological risk in the exposure of tadpoles of B. faber and L. latrans from the mixture of glyphosate + 2,4-D. Therefore, the approach used in this study provides important information on how commonly used pesticides can affect non-target organisms.

Automated summary

This study evaluates the chronic effects of glyphosate and 2,4-D mixture on Boana faber and Leptodactylus latrans tadpoles, finding that while the herbicide mixtures do not affect survival, they do alter growth and swimming activity, and cause damage in the mouth and intestine. The exposure to glyphosate and 2,4-D poses an ecological risk to tadpoles, highlighting the importance of understanding how commonly used pesticides can impact non-target organisms.