期刊
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
卷 40, 期 9, 页码 2559-2570出版社
WILEY
DOI: 10.1002/etc.5143
关键词
Avian toxicology; Avian endocrine disruption; RNA-Seq; Sex; Developmental stage
资金
- US Environmental Protection Agency (USEPA) [DW14922814-01-0]
- US Geological Survey, Contaminant Biology Program
- McGill Graduate Excellence Award program
- USEPA STAR program
The study found that male embryos had more differentially expressed genes after exposure to 17 beta-trenbolone compared to female embryos, while in adults, males and females had similar numbers of differentially expressed genes. Functional grouping and pathway enrichment analysis identified affected biological pathways including metabolism, growth factor signaling, and bile acid biosynthesis.
Endocrine-disrupting chemicals can cause transcriptomic changes that may disrupt biological processes associated with reproductive function including metabolism, transport, and cell growth. We investigated effects from in ovo and dietary exposure to 17 beta-trenbolone (at 0, 1, and 10 ppm) on the Japanese quail (Coturnix japonica) hepatic transcriptome. Our objectives were to identify differentially expressed hepatic genes, assess perturbations of biological pathways, and examine sex- and developmental stage-related differences. The number of significantly differentially expressed genes was higher in embryos than in adults. Male embryos exhibited greater differential gene expression than female embryos, whereas in adults, males and females exhibited similar numbers of differentially expressed genes (>2-fold). Vitellogenin and apovitellenin-1 were up-regulated in male adults exposed to 10 ppm 17 beta-trenbolone, and these birds also exhibited indications of immunomodulation. Functional grouping of differentially expressed genes identified processes including metabolism and transport of biomolecules, enzyme activity, and extracellular matrix interactions. Pathway enrichment analyses identified as perturbed peroxisome proliferator-activated receptor pathway, cardiac muscle contraction, gluconeogenesis, growth factor signaling, focal adhesion, and bile acid biosynthesis. One of the primary uses of 17 beta-trenbolone is that of a growth promoter, and these results identify effects on mechanistic pathways related to steroidogenesis, cell proliferation, differentiation, growth, and metabolism of lipids and proteins. Environ Toxicol Chem 2021;00:1-12. (c) 2021 SETAC. This article has been contributed to by US Government employees and their work is in the public domain in the USA.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据