期刊
ENVIRONMENTAL TOXICOLOGY
卷 36, 期 9, 页码 1944-1952出版社
WILEY
DOI: 10.1002/tox.23314
关键词
cell cycle; MEHP; neuroblastoma; Notch pathway; proliferation
资金
- Department of Finance of Jilin Province [JLSWSRCZX2020-090]
- Education Department of Jilin Province [JJKH20201118KJ, JJKH20201076KJ]
- National Natural Science Foundation of China [81972996]
MEHP exposure can promote neuroblastoma cell proliferation, alter the cell cycle, and affect cell apoptosis. The Notch pathway plays a critical role in this process.
Neuroblastoma (NB) is an estrogen-dependent tumor. Mono-2-ethylhexyl phthalate (MEHP) has an estrogen-like effect. However, the effects of MEHP on the progression of NB are not well illustrated. This study was to clarify the effect of Notch pathway on proliferation and cell cycle of SH-SY5Y cell induced by MEHP. The viability of SH-SY5Y and BE2C cells were detected by CCK8; cell cycle and apoptosis were detected by flow cytometry; the protein expression levels of Notch pathway and cell cycle related proteins were detected by Western-blot. Results show that MEHP exposure can promote cell proliferation and altered the cell cycle. MEHP exposure can up-regulate the expression of C-MYC, Cyclin D1, Bcl-2 and affected the Notch pathway. In conclusion, MEHP exposure can promote NB cell proliferation and affect the cell cycle and apoptosis. Notch pathway plays a critical role in accelerating the cell cycle and inhibiting the apoptosis of SH-SY5Y cells caused by MEHP.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据