Bioremediation assessment, hematological, and biochemical responses of the earthworm (Allolobophora caliginosa) in soil contaminated with crude oil

Soil contamination with crude oil is a major environmental problem. The aim of this study was to assess whether the earthworm Allolobophora caliginosa could improve the degradation of petroleum hydrocarbons while enriching soils that were contaminated with crude oil. In addition, the toxic effects of crude oil on earthworms during bioremediation will be assessed. The soil samples were experimentally contaminated with two different quantities of light crude oil 5 or 7.5 ml for 60 days. Activities of A. caliginosa resulted in 33.56% and 54.98% total petroleum hydrocarbon (TPH) losses from the soil contaminated with 5 ml of crude oil after 22 and 60 days; respectively. While in 7.5 ml crude oil-contaminated soil, there was a loss of 32.24% and 71.05% of TPH over the same period of time. During the experiment, however, there were no signs of improvement in soil physicochemical properties. Earthworm tissue analyses at the end of the experiment showed significant bioaccumulation of petroleum hydrocarbons in their tissues and changes in their metabolic and hematological parameters. Earthworms exposed to crude oil showed a significant increase in protein, malondialdehyde, and glutathione but decreased in catalase levels and total antioxidant capacity compared to control earthworm after 60 days of exposure. There was a significant decrease in the Hgb, RBCS, Hct, MCV, MCH, platelet count, and WBCs. As a result, the earthworm Allolobophora caliginosa has been shown to be good bioremediator for oil-contaminated soils and also has potential as a bioindicator for contamination.

Automated summary

The study found that the earthworm Allolobophora caliginosa has good bioremediation capabilities in oil-contaminated soil, but there was no significant improvement in the physicochemical properties of the soil during bioremediation. Additionally, earthworms exposed to crude oil experienced changes in metabolic and hematological parameters, significant bioaccumulation of petroleum hydrocarbons in their tissues, and alterations in certain oxidative stress markers.