Effect of protopine exposure on the physiology and gene expression in the bloom-forming cyanobacterium Microcystis aeruginosa

Environment-friendly sound measures with high algal growth inhibition efficiency are required to control and eliminate CyanoHABs. This study examined the effects of protopine on growth, gene expression, and antioxidant system of the M. aeruginosa TY001 and explored possible damage mechanism. The results revealed that higher concentrations of protopine seriously inhibited the growth of M. aeruginosa. Quantitative real-time PCR analysis showed downregulated expression of stress response genes (prx and fabZ), and DNA repair gene (recA) on days 3 and 5. The activities of antioxidant enzymes were also decreased markedly, including superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD). Additionally, protopine stress can significantly increase the malondialdehyde (MDA) level in cells. In conclusion, oxidative damage and DNA damage are the main mechanisms of protopine inhibition on M. aeruginosa TY001. Our studies provide evidence that alkaloid compounds such as protopine may have a potential use value as components of aquatic management strategies.

Automated summary

Protopine has been found to significantly inhibit the growth of M. aeruginosa TY001, mainly through downregulating stress response and DNA repair genes, reducing antioxidant enzyme activities, and increasing malondialdehyde levels in cells. These results suggest that protopine may have potential value in aquatic management strategies as a component that targets oxidative and DNA damage mechanisms in controlling and eliminating harmful algal blooms.