4.8 Article

Enhanced Secretions of Algal Cell-Adhesion Molecules and Metal Ion-Binding Exoproteins Promote Self-Flocculation of Chlorella sp. Cultivated in Municipal Wastewater

期刊

ENVIRONMENTAL SCIENCE & TECHNOLOGY
卷 55, 期 17, 页码 11916-11924

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acs.est.1c01324

关键词

microalgae; wastewater; harvesting; self-flocculation; extracellular polymeric substances; exoprotein

资金

  1. National Natural Science Foundation of China [52170038, 52000103]
  2. Fundamental Research Funds for the Central Universities [30921011219, 30920021117]
  3. Natural Science Foundation of Jiangsu Province for Distinguished Young Scholars [BK20190022]
  4. Natural Science Foundation of Jiangsu Province [BK20180497, BK20181303]
  5. Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX20_0329]
  6. Distinguished Professorship of Jiangsu Province, China
  7. China Association for Science and Technology

向作者/读者索取更多资源

This study highlights the crucial role of exoproteins in microalgal self-flocculation, showing that high light intensity conditions can enhance self-flocculation efficiency by promoting the enrichment and rearrangement of PN in EPS. Furthermore, specific PN over-expression, Algal-CAMs, and metal-ion-binding PN were identified as key contributors to facilitating cell adhesion and bridging.
The mechanism of self-flocculation remains unclear, partially impeding its efficiency enhancement and commercial application of microalgae-based municipal wastewater (MW) bioremediation technology. This study revealed the contributions of exoproteins [PN, proteins in extracellular polymeric substances (EPS)] to the separation of indigenous microalgae from treated MW. Compared to the low light intensity group, the high light intensity (HL) group produced Chlorella sp. with 4.3-fold higher self-flocculation efficiencies (SE). This was attributed to the enriched biological functions and positional rearrangement of increased PN within 2.9-fold higher EPS. Specifically, a total of 75 PN was over-expressed in the HL group among the 129 PN identified through label-free proteomics. The algal cell-adhesion molecules (Algal-CAMs) and metal-ion-binding PN were demonstrated as two dominant contributors promoting cell adhesion and bridging, through function prediction based on the contained domains. The modeled 3D structure showed that Algal-CAMs presented less hydrophilic a-helix abundance and were distributed in the outermost position of the EPS matrix, further facilitating microalgal separation. Moreover, the 10.1% lower hydrophily degree value, negative interfacial free energy (-19.5 mJ/m(2)), and 6.8-fold lower energy barrier between cells also supported the observed higher SE. This finding is expected to further fill the knowledge gap of the role of PN in microalgal self-flocculation and promote the development of biomass recovery from the microalgae-wastewater system.

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