期刊
FEBS LETTERS
卷 590, 期 16, 页码 2611-2618出版社
WILEY-BLACKWELL
DOI: 10.1002/1873-3468.12290
关键词
P-31 NMR; carbohydrate esterase; lignin-carbohydrate complexes; size exclusion chromatography; spruce and birch
资金
- Knut and Alice Wallenberg Foundation through the Wallenberg Wood Science Center (WWSC)
The Glucuronoyl esterases (GE) have been proposed to target lignin-carbohydrate (LC) ester bonds between lignin moieties and glucuronic acid side groups of xylan, but to date, no direct observations of enzymatic cleavage on native LC ester bonds have been demonstrated. In the present investigation, LCC fractions from spruce and birch were treated with a recombinantly produced GE originating from Acremonium alcalophilum (AaGE1). A combination of size exclusion chromatography and P-31 NMR analyses of phosphitylated LCC samples, before and after AaGE1 treatment provided the first evidence for cleavage of the LC ester linkages existing in wood.
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