期刊
FEBS LETTERS
卷 590, 期 10, 页码 1438-1446出版社
WILEY-BLACKWELL
DOI: 10.1002/1873-3468.12190
关键词
BMP-2; bone and cartilage development; embryos; microRNA-140-5p; zebrafish
资金
- Science and Technology Department of Hunan Province [2013JJ6037, 2014FJ2006]
- Scientific Research Fund of Hunan Provincial Education Department [14B114, 15CY006]
- Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province [20134486]
MicroRNA-140-5p (miRNA-140-5p) is important for embryonic bone development. In this study, we found that miRNA-140-5p and its binding site in the 3UTR of bone morphogenetic protein 2 (BMP-2) are highly conserved among vertebrates, and miRNA-140-5p negatively regulates both zebrafish and human BMP-2 genes. Microinjection of miRNA-140-5p or BMP-2b morpholino into zebrafish embryos led to a similar phenotype, including shortened tails, curved trunks, and defects in cranial cartilage. Moreover, miRNA-140-5p injection induced zebrafish embryo malformation that could be significantly rescued by microinjection of BMP-2 mRNA. In conclusion, our results indicated that miRNA-140-5p regulates zebrafish embryonic bone development via targeting BMP-2.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据