期刊
FEBS LETTERS
卷 590, 期 3, 页码 340-348出版社
WILEY-BLACKWELL
DOI: 10.1002/1873-3468.12060
关键词
autocleavage; deubiquitinating enzyme; DNA interstand crosslink repair; Fancd2; PCNA; Usp1
资金
- Chonnam National University
- National Research Foundation of Korea (Medical Research Council for Gene Regulation) - Ministry of Science, ICT and Future Planning [20110030132]
The Fanconi anemia (FA) pathway regulates DNA interstrand crosslink (ICL) repair. A critical step in this pathway is mono-ubiquitination of FANCD2 (FANCD2-Ub). Deubiquitinase USP1 removes ubiquitin from FANCD2 resulting in inactivation of the FA pathway. USP1 is autocleaved and subsequently degraded for its down-regulation. Here, we investigated the functional consequences of Usp1-autocleavage defect. Usp1-autocleavage mutant (Usp1 GG/AA) corrected the level of Fancd2-Ub similar to Usp1(WT) in Usp1(-/-) MEFs. However, Usp1 GG/AA only partially rescued MMC sensitivity with defective Fancd2 foci formation and homologous recombination defect. Contrary to this, Usp1 GG/AA was capable of recovering UV resistance of Usp1(-/-) MEFs to a similar extent with Usp1(WT). Taken together, our findings suggest that Usp1 regulation by autocleavage is critical for Usp1 to exert its function in ICL repair.
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