Cell surface engineering of Saccharomyces cerevisiae for simultaneous valorization of corn cob and cheese whey via ethanol production

The viability of 2nd generation bioethanol processes is dependent on achieving high ethanol titers, which requires the use of high solid loadings that will negatively affect the fermentative microorganism besides increasing enzyme-associated costs. To solve this, and also problems of feedstock availability, lignocellulosic biomass can be mixed with dairy by-products to increase carbon content. In this study, industrial strains of Saccharomyces cerevisiae, with improved thermotolerance and stress resistance, were engineered for the cell surface display of cellulolytic enzymes and were evaluated in consolidated bioprocessing of cellulose. Additionally, beta-galactosidase was also displayed to enable lactose consumption, resulting in high ethanol titers (>50 g/L) from the simultaneous use of cheese whey and pretreated corn cob as substrate. The multi-feedstock valorization approach together with this lactose-consuming cellulolytic yeast allowed the reduction on materials costs by 60% with a 2.5-fold increase in the annual ethanol production, therefore contributing to the establishment of economic viable ethanol processes.

Automated summary

This study engineered industrial strains of Saccharomyces cerevisiae with improved thermotolerance and stress resistance for cell surface display of cellulolytic enzymes, enabling high ethanol titers through consolidated bioprocessing. Additionally, beta-galactosidase was displayed for lactose consumption, resulting in high ethanol production from the simultaneous use of cheese whey and pretreated corn cob as substrate. The multi-feedstock valorization approach and lactose-consuming cellulolytic yeast led to a 60% reduction in materials costs and a 2.5-fold increase in annual ethanol production, contributing to the establishment of economically viable ethanol processes.