4.5 Article

Fabrication of a Sensitive and Stable NiO Uric Acid Biosensor Using Ag Nanowires and Reduced Graphene Oxide

期刊

ENERGIES
卷 14, 期 15, 页码 -

出版社

MDPI
DOI: 10.3390/en14154696

关键词

uric acid biosensor; nickel oxide (NiO); reduced graphene oxide (rGO); silver nanowires (AgNWs)

资金

  1. Ministry of Science and Technology, Taiwan, Republic of China [MOST 109-2221-E-224-013, MOST 110-2221-E-224-051]

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This study prepared a potentiometric uric acid biosensor using nickel oxide (NiO), reduced graphene oxide (rGO), and silver (Ag) nanowires, showing good sensitivity and stability for detecting uric acid.
How to detect uric acid is an important issue. For the purpose of preparing a potentiometric uric acid biosensor, this research used nickel oxide (NiO) as the sensing film to deposit it onto the substrate by radio frequency sputtering, then modified it with reduced graphene oxide (rGO) and silver (Ag) nanowires. Reduced graphene oxide (rGO) not only has excellent electrical conductivity, but also can make the surface of the film have a larger surface area, while AgNWs have also been proven to improve catalytic activity; hence, these two materials were chosen as sensor modifiers. Finally, the stability and the various characteristics of the uric acid biosensor were investigated using a voltage-time (V-T) system. The results showed that the AgNW-uricase/rGO/NiO uric acid biosensor has average sensitivity with 4.66 mV/(mg/L). In addition, the sensor has good stability.

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