期刊
ENDOCRINOLOGY
卷 162, 期 12, 页码 -出版社
ENDOCRINE SOC
DOI: 10.1210/endocr/bqab178
关键词
O-GlcNAcylation; pituitary adenoma; POMC; CRH; Cushing disease; corticotrope; mRNA decay; mRNA splicing
资金
- National Institute of Child Health and Development [R00HD087430]
This study highlights the significant role of O-GlcNAcylation in the pathophysiology of ACTH-secreting adenomas, including cellular proliferation and hypersecretion. O-GlcNAc enzymes were found to be upregulated in aggressive tumors, indicating a potential link between O-GlcNAcylation and pituitary adenoma etiology. Additionally, O-GlcNAcylation was shown to play a crucial role in the endocrine function of pituitary adenomas, particularly in corticotropic tumors.
Pituitary adenomas have a staggering 16.7% lifetime prevalence and can be devastating in many patients because of profound endocrine and neurologic dysfunction. To date, no clear genomic or epigenomic markers correlate with their onset or severity. Herein, we investigate the impact of the O-GlcNAc posttranslational modification in their etiology. Found in more than 7000 human proteins to date, O-GlcNAcylation dynamically regulates proteins in critical signaling pathways, and its deregulation is involved in cancer progression and endocrine diseases such as diabetes. In this study, we demonstrated that O-GlcNAc enzymes were upregulated, particularly in aggressive adrenocorticotropin (ACTH)-secreting tumors, suggesting a role for O-GlcNAcylation in pituitary adenoma etiology. In addition to the demonstration that O-GlcNAcylation was essential for their proliferation, we showed that the endocrine function of pituitary adenoma is also dependent on O-GlcNAcylation. In corticotropic tumors, hypersecretion of the proopiomelanocortin (POMC)-derived hormone ACTH leads to Cushing disease, materialized by severe endocrine disruption and increased mortality. We demonstrated that Pomc messenger RNA is stabilized in an O-GlcNAc-dependent manner in response to corticotrophin-releasing hormone (CRH). By affecting Pomc mRNA splicing and stability, O-GlcNAcylation contributes to this new mechanism of fast hormonal response in corticotropes. Thus, this study stresses the essential role of O-GlcNAcylation in ACTH-secreting adenomas' pathophysiology, including cellular proliferation and hypersecretion.
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