Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture

Nitrate commands genome-wide gene expression changes that impact metabolism, physiology, plant growth, and development. In an effort to identify new components involved in nitrate responses in plants, we analyze the Arabidopsis thaliana root phosphoproteome in response to nitrate treatments via liquid chromatography coupled to tandem mass spectrometry. 176 phosphoproteins show significant changes at 5 or 20 min after nitrate treatments. Proteins identified by 5 min include signaling components such as kinases or transcription factors. In contrast, by 20 min, proteins identified were associated with transporter activity or hormone metabolism functions, among others. The phosphorylation profile of NITRATE TRANSPORTER 1.1 (NRT1.1) mutant plants was significantly altered as compared to wild-type plants, confirming its key role in nitrate signaling pathways that involves phosphorylation changes. Integrative bioinformatics analysis highlights auxin transport as an important mechanism modulated by nitrate signaling at the post-translational level. We validated a new phosphorylation site in PIN2 and provide evidence that it functions in primary and lateral root growth responses to nitrate.

Automated summary

Nitrate induces genome-wide gene expression changes that affect plant metabolism, physiology, growth, and development. Analysis of the Arabidopsis thaliana root phosphoproteome in response to nitrate treatments identified 176 phosphoproteins with significant changes at 5 or 20 minutes, involving signaling components and transporter activity. The phosphorylation profile of NITRATE TRANSPORTER 1.1 mutant plants indicated its key role in nitrate signaling pathways, with auxin transport highlighted as a mechanism modulated by nitrate at the post-translational level for root growth responses.