期刊
EMBO REPORTS
卷 22, 期 7, 页码 -出版社
WILEY
DOI: 10.15252/embr.202051530
关键词
asymmetric cell division; Drosophila; epigenetics; FISH; histone
资金
- Howard Hughes Medical Institute [F31GM122339, F31DK122702, F31EY026786, R01EY025598, R01GM112008, R35GM127075]
- [55108512]
Stem cells can undergo asymmetric division to produce daughter cells with identical genes but different cell fates. In a study with Drosophila, it was found that daughter cells inherit histones differently at genes related to stem cell maintenance or differentiation during asymmetric division. This understanding may have implications for developing treatments for diseases related to cell fate abnormalities.
Stem cells have the unique ability to undergo asymmetric division which produces two daughter cells that are genetically identical, but commit to different cell fates. The loss of this balanced asymmetric outcome can lead to many diseases, including cancer and tissue dystrophy. Understanding this tightly regulated process is crucial in developing methods to treat these abnormalities. Here, we report that during a Drosophila female germline stem cell asymmetric division, the two daughter cells differentially inherit histones at key genes related to either maintaining the stem cell state or promoting differentiation, but not at constitutively active or silenced genes. We combine histone labeling with DNA Oligopaints to distinguish old versus new histones and visualize their inheritance patterns at a single-gene resolution in asymmetrically dividing cells in vivo. This strategy can be applied to other biological systems involving cell fate change during development or tissue homeostasis in multicellular organisms.
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