期刊
EMBO REPORTS
卷 22, 期 8, 页码 -出版社
WILEY
DOI: 10.15252/embr.202152905
关键词
chaperonin; ER stress; hematopoietic stem cell; planarian; starvation
资金
- Leibniz Institute on Aging-Fritz Lipmann Institute (FLI)
- Federal Government of Germany
- State of Thuringia
- German Research Foundation (DFG) as part of the PolyTarget collaborative research centre [CRC 1278]
- LGSA (Leibniz Graduate School on Ageing and Age Related Diseases) scholarship
- Projekt DEAL
Planarians can endure starvation by maintaining adult stem cell pools and regenerative capacity. TRiC/CCT subunits play a crucial role in maintaining regeneration under starvation conditions by modulating the unfolded protein response and maintaining ATP levels.
Planarians are able to stand long periods of starvation by maintaining adult stem cell pools and regenerative capacity. The molecular pathways that are needed for the maintenance of regeneration during starvation are not known. Here, we show that down-regulation of chaperonin TRiC/CCT subunits abrogates the regeneration capacity of planarians during starvation, but TRiC/CCT subunits are dispensable for regeneration in fed planarians. Under starvation, they are required to maintain mitotic fidelity and for blastema formation. We show that TRiC subunits modulate the unfolded protein response (UPR) and are required to maintain ATP levels in starved planarians. Regenerative defects in starved CCT-depleted planarians can be rescued by either chemical induction of mild endoplasmic reticulum stress, which leads to induction of the UPR, or by the supplementation of fatty acids. Together, these results indicate that CCT-dependent UPR induction promotes regeneration of planarians under food restriction.
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