期刊
EMBO JOURNAL
卷 40, 期 18, 页码 -出版社
WILEY
DOI: 10.15252/embj.2021107735
关键词
cilia; Cullin-RING ligase; Fbxw5; MCAK; ubiquitin
资金
- Deutsche Forschungsgemeinschaft [SPP1365, ME 2279/3, DFG KN590/7-1]
- European Union [748315]
- Heidelberg Cell Networks Cluster of Excellence postdoctoral programme
- Projekt DEAL
- Marie Curie Actions (MSCA) [748315] Funding Source: Marie Curie Actions (MSCA)
The ubiquitin E3 ligase SCFFbxw5 targets MCAK for proteasomal degradation during G(2), which promotes ciliogenesis starting in the preceding cell cycle phase. Loss of Fbxw5 results in increased MCAK levels at basal bodies and impairs ciliogenesis in the following G(1)/G(0).
Microtubule depolymerases of the kinesin-13 family play important roles in various cellular processes and are frequently overexpressed in different cancer types. Despite the importance of their correct abundance, remarkably little is known about how their levels are regulated in cells. Using comprehensive screening on protein microarrays, we identified 161 candidate substrates of the multi-subunit ubiquitin E3 ligase SCFFbxw5, including the kinesin-13 member Kif2c/MCAK. In vitro reconstitution assays demonstrate that MCAK and its closely related orthologs Kif2a and Kif2b become efficiently polyubiquitylated by neddylated SCFFbxw5 and Cdc34, without requiring preceding modifications. In cells, SCFFbxw5 targets MCAK for proteasomal degradation predominantly during G(2). While this seems largely dispensable for mitotic progression, loss of Fbxw5 leads to increased MCAK levels at basal bodies and impairs ciliogenesis in the following G(1)/G(0), which can be rescued by concomitant knockdown of MCAK, Kif2a or Kif2b. We thus propose a novel regulatory event of ciliogenesis that begins already within the G(2) phase of the preceding cell cycle.
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