期刊
EMBO JOURNAL
卷 40, 期 17, 页码 -出版社
WILEY
DOI: 10.15252/embj.2020105043
关键词
heat stress; intrinsically disordered regions; SnRK1; SNF1; AMPK; stress granules; tudor staphylococcal nuclease
资金
- European Commission [702473]
- Ministerio de Economia y Competitividad (Juan de la Cierva-Incorporacion) [IJCI-2016-30763]
- University of Seville [VIPPIT2020-IV. 4, VIPPIT2020-I.5]
- Ministerio de Economia y Competitividad [PGC2018-099048-B-100]
- Knut and Alice Wallenberg Foundation [2018.0026]
- Swedish Research Council VR [2019-04250_VR]
- Swedish Foundation for Strategic Research [RBP14-0037]
- Crops for the Future Research Programme
- Vinnova [2019-04250] Funding Source: Vinnova
- Swedish Foundation for Strategic Research (SSF) [RBP14-0037] Funding Source: Swedish Foundation for Strategic Research (SSF)
- Marie Curie Actions (MSCA) [702473] Funding Source: Marie Curie Actions (MSCA)
- Swedish Research Council [2019-04250] Funding Source: Swedish Research Council
TSN acts as a scaffold for stress granule proteins in plants, playing an important role in stress signaling and promoting the activation of SnRK1.
Tudor staphylococcal nuclease (TSN; also known as Tudor-SN, p100, or SND1) is a multifunctional, evolutionarily conserved regulator of gene expression, exhibiting cytoprotective activity in animals and plants and oncogenic activity in mammals. During stress, TSN stably associates with stress granules (SGs), in a poorly understood process. Here, we show that in the model plant Arabidopsis thaliana, TSN is an intrinsically disordered protein (IDP) acting as a scaffold for a large pool of other IDPs, enriched for conserved stress granule components as well as novel or plant-specific SG-localized proteins. While approximately 30% of TSN interactors are recruited to stress granules de novo upon stress perception, 70% form a protein-protein interaction network present before the onset of stress. Finally, we demonstrate that TSN and stress granule formation promote heat-induced activation of the evolutionarily conserved energy-sensing SNF1-related protein kinase 1 (SnRK1), the plant orthologue of mammalian AMP-activated protein kinase (AMPK). Our results establish TSN as a docking platform for stress granule proteins, with an important role in stress signalling.
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