Boronic Acid Substituted Polyaniline Based Enzymatic Biosensor System for Catechol Detection

In this research, an electrochemical enzymatic biosensor system for sensitive and selective catechol detection was developed with using boric acid substituted Polyaniline film coated two different electrodes; gold screen-printed electrode and glassy carbon electrode. Electropolymerization of 3-aminophenylboronic acid is carried out through various electropolymerization conditions, and the best conditions for biosensors systems are concluded. The developed tyrosinase enzyme-based biosensor system is used for the determination of catechol. The limit of detections(LOD) of the developed biosensor was 0.25 mu M and 1.8 mu M with Au SPE and GCE systems, respectively. Additionally, real sample analysis was also performed for controlled catechol added green tea samples.

Automated summary

In this research, an electrochemical enzymatic biosensor system utilizing boric acid substituted Polyaniline film was developed for sensitive and selective catechol detection. The best electropolymerization conditions for 3-aminophenylboronic acid were determined and the developed biosensor showed detection limits of 0.25 μM and 1.8 μM with Au SPE and GCE systems, respectively. Additionally, real sample analysis on green tea samples with controlled catechol added was conducted.