Monitoring of Intracellular Vesicles in Cultured Neurons at Different Growth Stages Using Intracellular Vesicle Electrochemical Cytometry

The exploration of intracellular vesicles plays crucial roles for neuronal activity assessment. Neurons at different growth stages may possess distinct neuronal activity, including vesicular content and release kinetics. Here, we monitored the vesicular content and its release kinetics in neurons at different growth stages by intracellular vesicle electrochemical cytometry. We found that the neurotransmitters content of vesicles changed to be increased and the vesicle release process became longer as the neurons grew. Further, we demonstrated that the vesicular adsorption and rupture modes changed from the dominant simple event to simple and complex event coexisting mode. We speculate that this work provides a new strategy for the neuronal activity assessment or real- time cell activity analysis.

Automated summary

By monitoring vesicular content and release kinetics in neurons at different growth stages using intracellular vesicle electrochemical cytometry, we observed an increase in neurotransmitter content and longer vesicle release process as neurons grew. Additionally, we found a shift in vesicular adsorption and rupture modes from dominant simple events to a coexistence of simple and complex events. This work may provide a new strategy for neuronal activity assessment and real-time cell activity analysis.